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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Rat Spleen Dendritic Cells Express Natural Killer Cell Receptor Protein 1 (NKR-P1) and Have Cytotoxic Activity to Select Targets via a Ca 2+ -dependent Mechanism
doi:
Figure Lengend Snippet: Upregulation of NKR-P1 expression on DC upon overnight in vitro culture. Enriched populations of fresh DC were prepared by selecting low-density cells of collagenase-digested spleen and thymus followed by magnetic beads depletion of non-DC. An aliquot of the cells was then analyzed in two-color immunofluorescence with biotynylated OX6 or OX62 mAbs followed by FITC-conjugated R7.3, 3.2.3, OX8, or control mAb and streptavidin-PE. The remaining cells were cultured overnight in complete RPMI and analyzed as described above. The histograms show expression patterns of TCR, NKR-P1, and CD8 on OX62-positive gated cells. Dotted lines show the background with isotype-matched control antibodies. The results are representative of four different experiments.
Article Snippet: For two-color immunofluorescence, 10 5 cells were incubated with
Techniques: Expressing, In Vitro, Magnetic Beads, Immunofluorescence, Control, Cell Culture